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Distinct gene expression pattern of mutations coordinated by target repression and promoter hypermethylation

Frontiers of Medicine 2022, Volume 16, Issue 4,   Pages 627-636 doi: 10.1007/s11684-020-0815-4

Abstract: Here, based on 1741 patients with AML, we report a unique expression pattern associated with RUNX1This expression pattern was coordinated by target repression and promoter hypermethylation.Then, based on RNA-seq data from The Cancer Genome Atlas AML cohort, we developed a 300-gene signatureThis study revealed the distinct gene expression pattern of RUNX1 mutations and the underlying

Keywords： RUNX1     gene mutation     acute myeloid leukemia     transcriptional repression     DNA methylation    

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MSI/LOH and extron expression of the FHIT gene in gastric carcinoma

XIAO Yuping, MAO Lili, HAN Chengbo, LI Jinyi, XU Lei, XIN Yan

Frontiers of Medicine 2007, Volume 1, Issue 1,   Pages 99-103 doi: 10.1007/s11684-007-0019-1

Abstract: We detected loss of heterozygosity (LOH) and microsatellite instabilities (MSI), as well as extron expressionof the fragile histidine triad (FHIT) gene in gastric carcinoma (GC), in order to evaluate their associationResults show that the average frequencies of LOH and MSI of the FHIT gene in GC were 32.4% and 26.4%,Four of 10 (40.0%) cases of primary GC showed absent or decreased expression of the FHIT protein as comparedThe findings in this study suggest that LOH and MSI of FHIT gene may induce aberrant extron expression
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Gene expression disparity in giant cell tumor of bone

Xiaohua PAN, Shuhua YANG, Deming XIAO, Yong DAI, Lili REN

Frontiers of Medicine 2009, Volume 3, Issue 1,   Pages 49-56 doi: 10.1007/s11684-009-0012-y

Abstract: The aim of this paper was to study the differential gene expression of giant cell tumor of bone (GCTB) by gene chip technology.The gene expression disparity between the GCTB samples and normal bony callus was significantly differentSpecific gene spectrum associated with GCTB can be identified by cDNA microarray, which will be the foundation

Keywords： giant cell tumor of bone     gene     microarray     cDNA    

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Construction of lentiviral vector carrying Rab9 gene and its expression in mouse brain

Youguo HAO, Min ZHANG, Jinzhi XU, Bitao BU, Jiajun WEI

Frontiers of Medicine 2009, Volume 3, Issue 2,   Pages 141-147 doi: 10.1007/s11684-009-0041-6

Abstract: To explore the possibility of Rab9-related gene therapy for neurodegenerative diseases, we packed LentivirusAfter 72 hours, the expression of GFP could be detected in BV-2 cells.Western blotting revealed that the Rab9 gene expression in BALB/c mice brain was up-regulated significantlyLentivirus encoding Rab9 can increase the expression of Rab9 gene effectively, which might offer a novel

Keywords： Rab9     lentivirus     gene therapy     gene transfer    

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A microarray study of altered gene expression during melanoblasts migration in normal pigmented White

Yulin LI,Deping HAN,Junying LI,Dawn KOLTES,Xuemei DENG

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 4,   Pages 299-306 doi: 10.15302/J-FASE-2014040

Abstract: vertebrates, melanocytes in Silky Fowl can reach the ventral regions of the embryos owing to differences in geneexpression in the process of melanoblasts migration.In this study, we used microarray profiling to identify differences in gene expression between WhiteDifferential expression of 2517 microarray probes ( <0.01, Fold Change>2) was observed in Silky FowlMoreover, differences in expression of immune genes were also detected between Silky Fowl and White Leghorn

Keywords： Silky Fowl     White Leghorn     melanoblast migration     gene expression    

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Optimized human factor IX expression cassettes for hepatic-directed gene therapy of hemophilia B

Ru Zhang,Qiang Wang,Lin Zhang,Saijuan Chen

Frontiers of Medicine 2015, Volume 9, Issue 1,   Pages 90-99 doi: 10.1007/s11684-015-0390-2

Abstract:

Gene therapy provides a potential cure for hemophilia B, and significant progress has been achievedin liver-directed gene transfer mediated by adeno-associated viral vectors.ultimate success of hemophilia B gene therapy.In addition, the regulatory elements that control the FIX gene expression in these cassettesreconstructs of hFIX with AAV vector may potentially contribute to the creation of an efficacious gene

Keywords： factor IX     hemophilia B     liver-specific regulatory elements     hydrodynamic gene transfer    

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Effects of different doses of cadmium on secondary metabolites and gene expression in Artemisia annua

Liangyun Zhou,Guang Yang,Haifeng Sun,Jinfu Tang,Jian Yang,Yizhan Wang,Thomas Avery Garran,Lanping Guo

Frontiers of Medicine 2017, Volume 11, Issue 1,   Pages 137-146 doi: 10.1007/s11684-016-0486-3

Abstract: The expression of key biosynthesis enzymes was also determined by qRT-PCR.The relative expression levels of HMGR, FPS, ADS, CYP71AV1the concentrations of artemisinic metabolites at a certain time point by up-regulating the relative expression

Keywords： Cd     secondary metabolites     gene expressions     Artemisia annua L.    

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Expression and bioinformatic analysis of lymphoma-associated novel gene KIAA0372

BAI Xiangyang, TANG Duozhuang, ZHU Tao, SUN Lishi, YAN Lingling, LU Yunping, ZHOU Jianfeng, MA Ding

Frontiers of Medicine 2007, Volume 1, Issue 1,   Pages 93-98 doi: 10.1007/s11684-007-0018-2

Abstract: lymphomas and reactive lymph node hyperplasia, and to perform an initial bioinformatic analysis on a novel genelymphomas and reactive lymph node hyperplasia were respectively marked with biotin and hybridized with GeneExpression Chips, resulting in differentially expressed genes.Initial bioinformatic analysis was then performed on a novel gene named KIAA0372, whose function hasUsing Gene Expression Chips, many differentially expressed genes were uncovered.

Keywords： bioinformatic analysis     functional     KIAA0372     detection     Microarray    

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Gene silencing efficiency of shRNA expression vectors targeting Cx43

Cuihong ZHENG, Yunxia WU, Guangying HUANG, Wei WANG

Frontiers of Medicine 2009, Volume 3, Issue 2,   Pages 130-135 doi: 10.1007/s11684-009-0030-9

Abstract: in acupuncture treatment, RNA interference technique was used to construct small hairpin RNA (shRNA) expressionDouble strands were formed after annealing, and then inserted into Pgenesil-1 plasmid expression vectorG418.The results of enzyme cutting and sequencing showed that we successfully constructed two shRNA expressionvectors targeting Cx43, and a control expression vector for rat and mouse.The outcomes demonstrate that the plasmid P-Cx43-shRNA (1) can specifically silence better the expression

Keywords： RNA interference     connexin 43     small hairpin RNA (shRNA)     acupuncture    

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Construction of eukaryotic expression vector of human arresten gene and its secreted expression in HEK

Wei LI PhD , Siming GUAN MM , Zifang SONG PhD , Qichang ZHENG PhD , Jun XIONG PhD , Dan SHANG PhD , Xiaogang SHU PhD ,

Frontiers of Medicine 2009, Volume 3, Issue 3,   Pages 297-302 doi: 10.1007/s11684-009-0058-x

Abstract: The eukaryotic expression vector of human arresten gene was constructed and its secretive expressionHuman arresten gene was amplified from recombinant plasmid pGEM-Arr by polymerase chain reaction (PCRThe target fragment was inserted into the I and I restriction sites of eukaryotic expression vectorrecombinant plasmid was subsequently transfected into HEK 293 cells with LipofectAMINETM2000 Reagent, and the expressionof the target gene was detected.

Keywords： angiogenesis inhibitor     arresten     eukaryotic expression     HEK 293 cells     endothelial cells    

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Construction of a universal recombinant expression vector that regulates the expression of human lysozyme

Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI

Frontiers of Agricultural Science and Engineering 2018, Volume 5, Issue 3,   Pages 382-389 doi: 10.15302/J-FASE-2018211

Abstract: A key component in the technology is the construction of an efficient milk expression vector.Here, we established a simple method to construct a milk expression vector, by a combination of homologousThe BAC of mouse whey acidic protein gene (mWAP) was modified twice by homologous recombinationto produce a universal expression vector, and the human lysozyme gene (hLZ) was then insertedthat the resulting vector regulates the expression of hLZ in milk.

Keywords： BAC recombinant methods     gene expression     human lysozyme     transgenic mice     milk expression vector    

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Correlation between cold and hot pattern in traditional Chinese medicine and gene expression profiles

Miao Jiang, Cheng Xiao, Gao Chen, Cheng Lu, Qinglin Zha, Xiaoping Yan, Weiping Kong, Shijie Xu, Dahong Ju, Pu Xu, Youwen Zou, Aiping Lu

Frontiers of Medicine 2011, Volume 5, Issue 2,   Pages 219-228 doi: 10.1007/s11684-011-0133-y

Abstract: In the present study, we combine genome-wide expression analysis with methods of systems biology to identifythe functional gene networks for the sets of clinical symptoms that comprise the major information forMicroarray technology was used to reveal gene expression profiles in CD4 T cells from 21 rheumatoidsignificant pathways and function were extracted from these subnetworks using the Biological Network Gene

Keywords： gene expression profile     pathway     rheumatoid arthritis     traditional Chinese medicine     systems biology    

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Influence and related mechanism of Retn gene expression on glucose uptake in 3T3-L1 cells

LI Yahui, DONG Shiyuan, YU Chao, JIANG Yu, LI Huaixing, SUN Shuhan

Frontiers of Medicine 2007, Volume 1, Issue 3,   Pages 269-273 doi: 10.1007/s11684-007-0051-1

Abstract: The aim of this article was to investigate the influence and the related mechanism of the gene on glucoseRadioimmunoassay was used to determine glucose uptake in 3T3-L1 cells with different gene expressionto determine the mRNA levels of several glucose transport proteins in 3T3-L1 cells with different geneexpression levels, including insulin receptor substrate-1(IRS-1), phosphatidylinositol 3-kinase (PI-The glucose uptake decreased with the increase in gene expression in 3T3-L1 cells, which was independent

Keywords： 3T3-L1     influence     resistance     receptor substrate-1     transport    

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Relative expression of PTTG and bFGF in oral squamous cell carcinoma and Tca8113

Yumei DING BM , Lili CHEN MD , Bo CHENG PhD , Handong ZHANG MM ,

Frontiers of Medicine 2009, Volume 3, Issue 3,   Pages 357-362 doi: 10.1007/s11684-009-0046-1

Abstract: The purpose of this study was to investigate the expression of pituitary tumor transforming gene (PTTGadditionally, after being treated with different concentrations of anti bFGF or PTTG antibody, PTTG or bFGF expressionPTTG protein expression was positively correlated with bFGF ( = 0.382, <0.05); (2) PTTG protein emittedAnti-PTTG antibody also had an inhibitive effect on bFGF expression.PTTG protein expression conforms to bFGF in OSCC tissues and Tca8113 cells.

Keywords： carcinoma     squamous cell     pituitary tumor transforming gene (PTTG) protein     basic fibroblast growth factor    

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effect of sodium perfluorononyloxy-benzenesulfonate on in aerobic denitrification, cell structure and geneexpression

Frontiers of Environmental Science & Engineering 2021, Volume 15, Issue 5, doi: 10.1007/s11783-021-1391-9

Abstract:

• OBS inhibited the growth of P. stutzeri and destroyed its structure.

Keywords： Sodium perfluorononyloxy-benzenesulfonate     Aerobic denitrification     Pseudomonas stutzeri     Ecotoxicity     ROS     Persist organic pollutants     Toxicity     Denitrification     Microbiology    

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Title Author Date Type Operation

Distinct gene expression pattern of mutations coordinated by target repression and promoter hypermethylation

Journal Article

MSI/LOH and extron expression of the FHIT gene in gastric carcinoma

XIAO Yuping, MAO Lili, HAN Chengbo, LI Jinyi, XU Lei, XIN Yan

Journal Article

Gene expression disparity in giant cell tumor of bone

Xiaohua PAN, Shuhua YANG, Deming XIAO, Yong DAI, Lili REN

Journal Article

Construction of lentiviral vector carrying Rab9 gene and its expression in mouse brain

Youguo HAO, Min ZHANG, Jinzhi XU, Bitao BU, Jiajun WEI

Journal Article

A microarray study of altered gene expression during melanoblasts migration in normal pigmented White

Yulin LI,Deping HAN,Junying LI,Dawn KOLTES,Xuemei DENG

Journal Article

Optimized human factor IX expression cassettes for hepatic-directed gene therapy of hemophilia B

Ru Zhang,Qiang Wang,Lin Zhang,Saijuan Chen

Journal Article

Effects of different doses of cadmium on secondary metabolites and gene expression in Artemisia annua

Liangyun Zhou,Guang Yang,Haifeng Sun,Jinfu Tang,Jian Yang,Yizhan Wang,Thomas Avery Garran,Lanping Guo

Journal Article

Expression and bioinformatic analysis of lymphoma-associated novel gene KIAA0372

BAI Xiangyang, TANG Duozhuang, ZHU Tao, SUN Lishi, YAN Lingling, LU Yunping, ZHOU Jianfeng, MA Ding

Journal Article

Gene silencing efficiency of shRNA expression vectors targeting Cx43

Cuihong ZHENG, Yunxia WU, Guangying HUANG, Wei WANG

Journal Article

Construction of eukaryotic expression vector of human arresten gene and its secreted expression in HEK

Wei LI PhD , Siming GUAN MM , Zifang SONG PhD , Qichang ZHENG PhD , Jun XIONG PhD , Dan SHANG PhD , Xiaogang SHU PhD ,

Journal Article

Construction of a universal recombinant expression vector that regulates the expression of human lysozyme

Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI

Journal Article

Correlation between cold and hot pattern in traditional Chinese medicine and gene expression profiles

Miao Jiang, Cheng Xiao, Gao Chen, Cheng Lu, Qinglin Zha, Xiaoping Yan, Weiping Kong, Shijie Xu, Dahong Ju, Pu Xu, Youwen Zou, Aiping Lu

Journal Article

Influence and related mechanism of Retn gene expression on glucose uptake in 3T3-L1 cells

LI Yahui, DONG Shiyuan, YU Chao, JIANG Yu, LI Huaixing, SUN Shuhan

Journal Article

Relative expression of PTTG and bFGF in oral squamous cell carcinoma and Tca8113

Yumei DING BM , Lili CHEN MD , Bo CHENG PhD , Handong ZHANG MM ,

Journal Article

effect of sodium perfluorononyloxy-benzenesulfonate on in aerobic denitrification, cell structure and geneexpression

Journal Article